The receptor binding domain of the new Middle East respiratory syndrome coronavirus maps to a 231-residue region in the spike protein that efficiently elicits neutralizing antibodies.
Identifieur interne : 001832 ( Main/Exploration ); précédent : 001831; suivant : 001833The receptor binding domain of the new Middle East respiratory syndrome coronavirus maps to a 231-residue region in the spike protein that efficiently elicits neutralizing antibodies.
Auteurs : Huihui Mou [Pays-Bas] ; V Stalin Raj ; Frank J M. Van Kuppeveld ; Peter J M. Rottier ; Bart L. Haagmans ; Berend Jan BoschSource :
- Journal of virology [ 1098-5514 ] ; 2013.
Descripteurs français
- KwdFr :
- Anticorps antiviraux (immunologie), Anticorps neutralisants (immunologie), Attachement viral, Coronavirus (immunologie), Coronavirus (physiologie), Dipeptidyl peptidase 4 (métabolisme), Déterminants antigéniques des lymphocytes B (génétique), Déterminants antigéniques des lymphocytes B (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (métabolisme), Humains, Lignée cellulaire, Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (métabolisme), Récepteurs viraux (métabolisme), Sites de fixation.
- MESH :
- génétique : Déterminants antigéniques des lymphocytes B.
- immunologie : Anticorps antiviraux, Anticorps neutralisants, Coronavirus, Déterminants antigéniques des lymphocytes B, Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- métabolisme : Dipeptidyl peptidase 4, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux.
- physiologie : Coronavirus.
- Attachement viral, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Sites de fixation.
English descriptors
- KwdEn :
- Antibodies, Neutralizing (immunology), Antibodies, Viral (immunology), Binding Sites, Cell Line, Coronavirus (immunology), Coronavirus (physiology), Dipeptidyl Peptidase 4 (metabolism), Epitopes, B-Lymphocyte (genetics), Epitopes, B-Lymphocyte (immunology), Humans, Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Receptors, Virus (metabolism), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism), Virus Attachment.
- MESH :
- chemical , genetics : Epitopes, B-Lymphocyte.
- chemical , immunology : Antibodies, Neutralizing, Antibodies, Viral, Epitopes, B-Lymphocyte, Membrane Glycoproteins, Viral Envelope Proteins.
- immunology : Coronavirus.
- chemical , metabolism : Dipeptidyl Peptidase 4, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- physiology : Coronavirus.
- Binding Sites, Cell Line, Humans, Spike Glycoprotein, Coronavirus, Virus Attachment.
Abstract
The spike (S) protein of the recently emerged human Middle East respiratory syndrome coronavirus (MERS-CoV) mediates infection by binding to the cellular receptor dipeptidyl peptidase 4 (DPP4). Here we mapped the receptor binding domain in the S protein to a 231-amino-acid fragment (residues 358 to 588) by evaluating the interaction of spike truncation variants with receptor-expressing cells and soluble DPP4. Antibodies to this domain--much less so those to the preceding N-terminal region--efficiently neutralize MERS-CoV infection.
DOI: 10.1128/JVI.01277-13
PubMed: 23785207
Affiliations:
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Le document en format XML
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Here we mapped the receptor binding domain in the S protein to a 231-amino-acid fragment (residues 358 to 588) by evaluating the interaction of spike truncation variants with receptor-expressing cells and soluble DPP4. Antibodies to this domain--much less so those to the preceding N-terminal region--efficiently neutralize MERS-CoV infection.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country><region><li>Utrecht (province)</li></region><settlement><li>Utrecht</li></settlement><orgName><li>Université d'Utrecht</li></orgName></list><tree><noCountry><name sortKey="Bosch, Berend Jan" sort="Bosch, Berend Jan" uniqKey="Bosch B" first="Berend Jan" last="Bosch">Berend Jan Bosch</name><name sortKey="Haagmans, Bart L" sort="Haagmans, Bart L" uniqKey="Haagmans B" first="Bart L" last="Haagmans">Bart L. Haagmans</name><name sortKey="Raj, V Stalin" sort="Raj, V Stalin" uniqKey="Raj V" first="V Stalin" last="Raj">V Stalin Raj</name><name sortKey="Rottier, Peter J M" sort="Rottier, Peter J M" uniqKey="Rottier P" first="Peter J M" last="Rottier">Peter J M. Rottier</name><name sortKey="Van Kuppeveld, Frank J M" sort="Van Kuppeveld, Frank J M" uniqKey="Van Kuppeveld F" first="Frank J M" last="Van Kuppeveld">Frank J M. Van Kuppeveld</name></noCountry><country name="Pays-Bas"><region name="Utrecht (province)"><name sortKey="Mou, Huihui" sort="Mou, Huihui" uniqKey="Mou H" first="Huihui" last="Mou">Huihui Mou</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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